Beta-lactamase database (BLDB) – structure and function
نویسندگان
چکیده
منابع مشابه
Probing beta-lactamase structure and function using random replacement mutagenesis.
A new analytical mutagenesis technique is described that involves randomizing the DNA sequence of a short stretch of a gene (3-6 codons) and determining the percentage of all possible random sequences that produce a functional protein. A low percentage of functional random sequences in a complete library of random substitutions indicates that the region mutagenized is important for the structur...
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Site-directed mutagenesis of Ser-289 of the class C beta-lactamase from Enterobacter cloacae P99 was performed to investigate the role of this residue in beta-lactam hydrolysis. This amino acid lies near the active site of the enzyme, where it can interact with the C-3 substituent of cephalosporins. Kinetic analysis of six mutant beta-lactamases with five cephalosporins showed that Ser-289 can ...
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We report the crystal structure of a class D beta-lactamase, the broad spectrum enzyme OXA-10 from Pseudomonas aeruginosa at 2.0 A resolution. There are significant differences between the overall fold observed in this structure and those of the evolutionarily related class A and class C beta-lactamases. Furthermore, the structure suggests the unique, cation mediated formation of a homodimer. K...
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The Structure-Function Linkage Database (SFLD, http://sfld.rbvi.ucsf.edu/) is a manually curated classification resource describing structure-function relationships for functionally diverse enzyme superfamilies. Members of such superfamilies are diverse in their overall reactions yet share a common ancestor and some conserved active site features associated with conserved functional attributes ...
متن کاملPatterns Structure Database Structure Motif Database Structure Description Extract Match Structure Prediction Function
This article investigate diierent aspects regarding pairwise and multiple structure comparison , and the problem of automatically discover common patterns in a set of structures. Descriptions and representation of structures and patterns are investigated, as well as scoring and algorithms for comparison and discovery. A framework and nomenclature is developed, and a lot of methods are reviewed ...
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ژورنال
عنوان ژورنال: Journal of Enzyme Inhibition and Medicinal Chemistry
سال: 2017
ISSN: 1475-6366,1475-6374
DOI: 10.1080/14756366.2017.1344235